Utilization of Non-specific Nitrogen Sources

نویسندگان

  • DOUGLAS V. FROST
  • HARRY R. SANDY
چکیده

The rat repletion method of assay of protein value, developed by Cannon’s group at the University of Chicago (24), has been applied to liquid protein hydrolysates and amino acid mixtures in this laboratory (5, 6). The method has the advantage of convenient volumetric portioning of the amino acid components of the diet. With properly conditioned depleted animals, there is quantitative acceptance of liquid protein hydrolysates of high biologic value over a wide range of nitrogen intake; i.e., up to 400 mg. of N per rat day. Whole, dry, powdered proteins fed in cups separate from the non-protein diet are also quantitatively consumed, and provide convenient standards of protein value for comparison with amino acid solutions (7). In the present studies, solutions of ‘mixtures of pure amino acids were generally fed at a level to supply 100 to 120 mg. of available N per rat day. This is equivalent to approximately 1 per cent of N in the diet, or only about 6 to 7 per cent of protein. This level is well below the minimum level at which maximum growth responses are obtained even with proteins of high biologic value such as lactalbumin, whole egg, and fibrin, and is, therefore, highly critical with regard to limit.ing deficiencies of individual amino acids. Rose (8) suggested many years ago, and reemphasized recently, that it may be possible with the exact knowledge of individual essential amino acid requirements to devise mixtures of extraordinary value for parenteral hse. Such mixtures must obviously contain those eight amino acids which Rose has shown essential for maintenance of N balance in adult men. In addition, one must consider appropriate sources of nitrogen to supply the body needs for the non-essential amino acids plus other nitrogen metabolites ordinarily supplied by food. The eight essential amino acids account for only 40 to 50 per cent of the composition of proteins of the highest biologic value. The proteins of lower biologic value have an even lesser ratio of the essential to non-essential amino acids. The question arises, then, as to the optimum nutritive ratio between essential and non-essential

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تاریخ انتشار 2003